- 14-17 SEP 2023
- Riyadh, Saudi Arabia
| Call for Abstracts: | 20 July 2023 |
| Deadline of Abstract Submission: | 01 September 2023 |
| Conference Date: | 14-17 September 2023 |
Location:
Crown Plaza RDC, Riyadh, Saudi Arabia.
Awards:
Awards will be given to the top 3 abstracts per category (senior and young investigators) and paper of the year chosen by the Scientific Committee.
Abstract Topics:
Who Should Attend?
Any researcher working or studying in Saudi Arabia is invited to submit his/her hematology related research (Physicians, Pathologists, Scientists, Trainees and Students).
Submission Procedure:
Abstracts shall be submitted via abstract page at the conference website to: ssbdconference.org.
Rules and Regulations:
Selection Criteria:
Abstract Format:
Abstracts should be limited to a maximum of 1000 words.
All abstracts must include the following: (Please refer to the attached template)
Presentation
Oral presentation or poster presentation.
ABSTRACT SUBMISSION DEADLINE:
01st September 2023
Please note that you must organize and submit your abstract using this template. Please do not use all CAPS. Please submit your abstract by uploading file in WORD FORMAT to abstract page at conference site
ssbdconference.org
ABSTRACT BODY
The abstract must not exceed 1000 words (including tables). Your abstract must include a title, list of authors and affiliations, and content including a concise introduction, statement of the main thesis, summary and conclusion. The format for trial/study abstracts should include the following sections: objective, rationale, methodology, results and conclusions.
Characterizing non-hematopoietic progenitor cell populations from human umbilical cord blood: implications for cord blood banking
Halpenny M 1, Yang L1, Birch P 1(Presenter), Martin L1, Fung Kee Fung K 2, Haebe J 2, Li C3, Allan DS 3, Giulivi A 1, Goldman M1
1The Canadian Blood Services Stem Cell Processing Laboratory, Ottawa; 2Department of Obstetrics, The Ottawa Hospital, and 3 Ottawa Health Research Institute
Background: Banking of human umbilical cord blood is increasing worldwide due to the increasing need to find alternative stem cell sources for hematopoietic transplantation and for their emerging role in regenerative therapy. The Canadian Blood Services Ottawa Stem Cell laboratory provides stem cell processing services to multiple clinical sites, is FACT-accredited, and processes human stem cell products in accordance with GMP guidelines. In anticipation of an increasing role of Canadian Blood Services in cord blood banking, we sought to characterize progenitor cell populations in human umbilical cord blood.
Methods: Following informed consent, human umbilical cord blood (UCB) was collected following vaginal delivery or elective Caesarean section using a commercially available cord blood collection kit. CBUs were transported from The Ottawa Hospital to Canadian Blood Services in standard boxes at ambient temperature and processed within 24 hours of collection. Mononuclear cells were isolated, enumerated and cultured using previously published methods to characterize mesenchymal stem cells (MSC), endothelial-like vascular progenitor cells (VPC) (Endocult, Stem Cell Technologies), hematopoietic stem cells colony-forming units (Methocult, Stem Cell Technologies), and neural stem cells (NSC) (Stem Cell Technologies). In addition, a bioarchiving and cryopreservation system was designed to provide a reliable inventory database to track all research samples from laboratory freezers to final disposition.
Results: Cord blood samples (n=12) were processed according to our standard protocol. All samples
yielded CFU , CFU and CFU
………………Column Break………………. Samples (n=4/12) also yielded VPC clusters which were
E GM, GEMM
CD45+CD14+ and VEGFR2+. In addition, cells/colonies with MSC morphology and immunophenotype (CD90+, CD105+ and CD34/45-) were isolated (n=2/4). These UCB MSC-like cells, could undergo multiple passages and differenciate into different connective tissue lineages such as bone, carilage, and adipose tissues.
Conclusion: Research CBUs can be effectively collected, transported, processed and stored safely in a clinical stem cell laboratory using standard protocols and a bioarchive system. Initial cord blood units processed at our facility retain hematopoietic characteristics required for hematopoietic stem cell transplan